Women are increasingly taking part in the business market, especially in the case of tourism. However, studies reveal that entrepreneurial women, besides the business, have other tasks, such as domestic and family obligations, being the majority responsible for the family management. The main purpose of this chapter is to investigate the contribution of entrepreneurial women in tourism companies in rural areas. A survey was applied, through a semi-structured interview, to a total of 13 tourism companies located in the municipality of Sever do Vouga (Portugal). The results indicate that although women are fewer and working together with their husbands, they are already retired, which facilitates dedication and commitment to the business on a full-time basis. Younger women with children may encounter barriers that make it harder for them to dedicate themselves to the enterprise. This research contributes to a better definition of the profile and behaviour of entrepreneurial women in rural areas, as well as identifying the motivations and barriers to invest in rural tourism.